湖南生态科学学报

【目的】探讨龙牙百合与不同百合种及品种间的杂交亲和性，以期培育出优良的龙牙百合杂交后代(赏食兼用百合新品种)。【方法】将龙牙百合与同为食用百合的兰州百合、山丹，以及观赏价值高、抗性强的栽培品种‘索邦’‘乌西达’进行了远缘杂交，采用盛花期重复授粉克服受精前的障碍，利用胚拯救克服受精后的障碍，并设置不同1-萘乙酸(NAA)质量浓度(0.30 mg/L、0.50 mg/L、0.70 mg/L)对杂种后代进行了增殖培养。【结果】龙牙百合与4个百合父本都获得了杂交蒴果，其中，与‘索邦’杂交结实率最高，为29.17%。通过胚拯救技术，4个杂交组合均获得杂种苗，其中，龙牙百合与‘索邦’杂交获得的杂种苗数量最多，共获得59株杂种苗，成苗率为5.72%。【结论】龙牙百合与‘索邦’杂交的受精前障碍和受精后障碍均较小，亲和性最高。在杂交后代增殖培养时，龙牙百合与‘索邦’在添加NAA浓度为0.50 mg/L时增殖情况最佳，为今后培育赏食兼用的百合新品种提供了理论和材料基础。

【Objective】Investigating the hybrid compatibility between Lilium brownii var. Viridulum Baker and various lily species and cultivars to develop superior hybrid progeny of Lilium brownii var. Viridulum Baker(new ornamental and edible lily varieties).【Methods】Lilium brownii var. Viridulum Baker was crossbred with other edible species(Lilium davidii var. unicolor; Lilium pumilum) as well as ornamental cultivars with high aesthetic value and strong stress resistance(Lilium oriental hybrids 'Sorbonne' and Lilium 'Uchida'). Pre-fertilization barriers were overcome by repeated pollination during peak flowering, while post-fertilization barriers were addressed using embryo rescue technology. Different NAA concentrations(0.30 mg/L, 0.50 mg/L, and 0.70 mg/L) were tested for proliferation culture of hybrid progenies.【Results】Lilium brownii var. Viridulum Baker×Lilium and the four male parents all produced hybrid capsules, with the highest fruit set rate of 29.17% observed in the cross with 'Sorbonne'. Embryo rescue successfully produced hybrid seedlings in all four cross combinations, with Lilium brownii var. Viridulum Baker×Lilium oriental hybrids 'Sorbonne' generating the maximum of 59 seedlings(5.72% seedling rate).【Conclusion】Both pre-fertilization and post-fertilization barriers in the hybrid between Lilium brownii var. Viridulum Baker and 'Sorbonne' are relatively low, exhibiting the highest level of compatibility. Optimal proliferation conditions were achieved at 0.50 mg/L NAA for Lilium brownii var. Viridulum Baker×Lilium oriental hybrids 'Sorbonne'. The study establishes both theoretical and material foundations for developing novel dual-purpose lily varieties.