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【目的】观察多年生牧草种植对喀斯特地区土壤球囊霉素相关土壤蛋白(GRSP)含量及其对土壤有机碳(SOC)固持的影响，为喀斯特地区草地土壤团聚体形成机制提供新的数据支持，并为优化草地利用与管理提供科学参考。【方法】在桂西北典型喀斯特峰丛洼地区选择14处人工牧草地及其邻近玉米地，分别采集表层(0～15 cm)和亚表层(30～45 cm)土壤样品，测定总提取球囊霉素相关土壤蛋白(T-GRSP)和易提取球囊霉素相关土壤蛋白(EE-GRSP)含量，计算其在土壤有机碳(SOC)中的占比，并利用逐步回归分析探讨其主要影响因素。【结果】两种土壤利用方式下，牧草地表层与亚表层的T-GRSP与EE-GRSP较玉米地均提高(P<0.05),而EE-GRSP/SOC、T-GRSP/SOC均下降(P<0.05)。主控因子呈土层分异，表层以全氮(TN)、全磷(TP)与土壤容重(BD)为主，EE-GRSP/T-GRSP受pH独立调节；亚表层EE-GRSP/SOC主要受土壤微生物生物量碳(MBC)控制，T-GRS/SOC中的占比主要受TN影响。【结论】在西南喀斯特峰丛洼区，耕地改种多年生牧草显著提高土壤了GRSP含量，表层增幅高于亚表层，其原因是耕作扰动降低与根系为丛枝菌根真菌(AMF)提供宿主与碳源；然而GRSP/SOC下降，亚表层降幅更大。驱动具有土层分异：表层受全氮正向、全磷负向调控；亚表层受微生物生物量碳与全氮共同正向作用。
Abstract：【Objective】Understanding the impacts of establishing perennial pastures in karst regions on glomalin-related soil protein(GRSP) contents, soil aggregate formation, and the stabilization of soil organic carbon(SOC) provides new empirical evidence for the mechanisms of aggregate formation in karst grassland soils. It also offers a scientific basis for optimizing grassland use and management to achieve a win-win outcome of economic returns and development. 【Method】In the typical karst peak-cluster depression area of northwest Guangxi, 14 artificial grassland sites and adjacent cornfields were chosen. Soil samples were collected from the 0-15 cm and 30-45 cm depths. The contents of total glomalin-related soil protein(T-GRSP) and easily extractable glomalin-related soil protein(EE-GRSP) were measured. Stepwise regression analysis was employed to explore the primary factors influencing the proportion of GRSP in SOC.【Result】 Across the two land-use types, pasture soils had higher T-GRSP and EE-GRSP in both the topsoil and subsoil. However, the ratios of EE-GRSP/SOC and T-GRSP/SOC decreased. The dominant controlling factors were depth-stratified. In the surface layer, total nitrogen(TN), total phosphorus(TP), and bulk density(BD) were primary drivers, and the EE-GRSP/T-GRSP ratio was independently regulated by pH. In the subsurface layer, EE-GRSP/SOC was mainly governed by microbial biomass carbon(MBC), and TN primarily influenced the T-GRSP/SOC proportion. 【Conclusion】 In the southwest karst peak-cluster depression region, converting cropland to perennial pasture significantly increased soil GRSP, with greater increases in the topsoil than in the subsoil. This can be attributed to reduced tillage disturbance and pasture roots providing hosts and carbon for arbuscular mycorrhizal fungi(AMF). However, the GRSP/SOC ratio decreased, with a more substantial decline in the subsoil. The controlling factors were depth-dependent: in the topsoil, GRSP was positively regulated by TN and negatively by TP, while in the subsoil, MBC and TN acted jointly and positively.
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基本信息:
中图分类号:S812.2
引用信息:
[1]谢佳轩,肖孔操,杨君,等.喀斯特峰丛洼地区牧草种植对球囊霉素相关土壤蛋白含量的影响[J].湖南生态科学学报,2025,12(03):8-17.
基金信息:
国家自然科学基金(U21A2007); 中国科学院西部之光项目(U21A2007)